New EPIC nuclear DNA sequence markers to improve the resolution of phylogeographic studies of coenagrionids and other odonates

While phylogeographic data provide valuable information to inform conservation plans, there are comparatively few Odonata phylogeographic studies. This lack of research is partially due to a lack of independent DNA markers with appropriate levels of polymorphism that PCR-amplify in a range of species. We followed an exon-primed, intron-crossing (EPIC) PCR strategy to develop five new,…

Phoenicagrion trilobum, a new species of damselfly from Peru (Odonata: Coenagrionidae)

Three species of Phoenicagrion are known from the north-eastern Amazonian part of Peru: P. flammeum, P. paulsoni, and a third undescribed species. In 2009 and 2010 several specimens of this third species were collected in Loreto, making it possible to describe this species, here named Phoenicagrion trilobum (holotype ♂: Peru, Loreto department, Tahuayo River, 18 km E…

An examination of competitive gametic isolation mechanisms between the damselflies Ischnura graellsii and I. elegans

Recent findings suggest that postmating prezygotic isolation (i.e. gametic barriers) could be an important factor preventing hybrid formation. Competitive gametic barriers emerge when a female is inseminated by a conspecific and a heterospecific male. We examined whether sperm proportions after double matings and copulation duration impede hybrid formation. For this, we used females of Ischnura…

Pyrrhosoma and its relatives: a phylogenetic study (Odonata: Zygoptera)

The placement and relationships of the red-and-black zygopteran Pyrrhosoma, currently considered to be part of the Teinobasinae, has long been uncertain. DNA fragments (COI and ITS) reveal that Pyrrhosoma s.s. is restricted to the West Palaearctic, with two morphologically distinct name-bearing clades (nymphula, elisabethae), and with a morphologically indistinct third clade in the Middle Atlas,…

Three new Cephalaeschna species from central China with descriptions of the hitherto unknown sex of related species (Odonata: Aeshnidae)

Three new Cephalaeschna species, C. discolor sp. nov. (holotype male; Shennongjia National Nature Reserve, Shennongjia City, Hubei province, China, 16 August 2012), C. mattii sp. nov. (holotype male; Lujiahe River, Zigui County, Hubei province, China, 18 September 2012) and C. solitaria sp. nov. (holotype male; Dalongtan in Shennongjia National Nature Reserve, Shennongjia City, Hubei province,…

Habitat segregation of sympatric Mnais damselflies (Odonata: Calopterygidae): microhabitat insolation preferences and competition for territorial space

Distribution and abundance of sympatric Mnais costalis and M. pruinosa damselflies were studied in a low mountain stream in Shiga, Japan, from 2008 through 2012. The reproductive seasons of the two species overlapped almost entirely: both species emerged in early May and disappeared in late June each year. Males of both species hold territories within…