New EPIC nuclear DNA sequence markers to improve the resolution of phylogeographic studies of coenagrionids and other odonates
International Journal of Odonatology, Volume 17, Issue 2-3, Pages 135-147, 2014
Published: 3 July 2014 (Received: 2 May 2014, Accepted: 25 July 2014)
While phylogeographic data provide valuable information to inform conservation plans, there are comparatively few Odonata phylogeographic studies. This lack of research is partially due to a lack of independent DNA markers with appropriate levels of polymorphism that PCR-amplify in a range of species. We followed an exon-primed, intron-crossing (EPIC) PCR strategy to develop five new, polymorphic nuclear DNA sequence loci (six distinct DNA fragments) for the southern damselfly Coenagrion mercuriale. These markers were: cell division cycle 5 protein (CDC5), arginine methyltransferase (PRMT), acetylglucosaminyl-transferase (AgT), myosin light chain (MLC) and phosphoglucose isomerase (PGI). Between three and five of these new markers could be PCR-amplified in five other species from the genus Coenagrion; one locus (PRMT) can be used in 26 other species of odonates that we examined, including three species of Anisoptera belonging to the genus Onychogomphus. These new nuclear genetic markers will be useful for phylogeographic studies in a range of odonate species, but also for phylogenetic studies, providing a particularly useful complement to the existing mitochondrial and nuclear loci.
Keywords: Coenagrionidae, Coenagrion mercuriale, dragonfly, genetic diversity, introns, multilocus approach, non-model organism, nuclear DNA loci, Odonata, phylogeny
Issue section: Article