Cross-species amplification of microsatellite loci in some European zygopteran species (Odonata: Coenagrionidae)

Phillip C. Wattsa, David J. Thompsona, Stephen J. Kempa

  1. School of Biological Sciences, The Biosciences Building, Crown Street, University of Liverpool, Liverpool, UK

International Journal of Odonatology, Volume 7, Issue 1, Pages 87-96, 2004
https://doi.org/10.1080/13887890.2004.9748198
Published: 1 April 2004 (Received: 23 October 2003, Accepted: 15 November 2003)

Abstract

Microsatellites have been infrequently used for genetic analysis of odonate species. Here, we report four microsatellite loci that are polymorphic in Coenagrion mercuriale. Furthermore, we examine the success of cross-species amplification of a panel of 19 microsatellite loci that were developed from C. mercuriale in seven other European odonate species. PCR with microsatellite primers is more likely to be achieved in species that are closely related to the species used for marker isolation. Overall, 10 microsatellite loci amplified interpretable PCR products (seven loci were variable) in both C. puella and C. pulchellum, whilst two loci were variable in Ischnura elegans. These markers should facilitate genetic research into these zygopteran species.

Keywords: Odonata, dragonfly, Coenagrion mercurial, C. puella, C. pulchellum, Ischnura elegans, Coenagrionidae, genetic variation, microsatellite

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