The Pseudagrion split: molecular phylogeny confirms the morphological and ecological dichotomy of Africa’s most diverse genus of Odonata (Coenagrionidae)

The continental African representatives of the genus Pseudagrion fall into two groups (A and B) based on their ecology and larval and adult morphology. While the B-group species are found in generally warmer habitats, which are more sunny, lentic or low-lying, the A-group representatives occur more in cooler habitats. We compared molecular genetic and ecological…

New fossil damselflies from Baltic Amber, with description of a new species, a redescription of Litheuphaea carpenteri Fraser, and a discussion on the phylogeny of Epallagidae (Zygotera: Caloptera)

Litheuphaea ludwigi sp. n. is described as first representative of Epallagidae from Baltic amber. The holotype of Litheuphaea carpenteri Fraser, 1955 is redescribed, the phylogenetic position of all fossil Epallagidae is discussed, and a new phylogenetic classification is proposed. The authorship of Selys (1853) for the family-group name Euphaeidae is rejected, since the “légion Euphaea”…

Distribution, autecology, genetic characterization, and conservation of the Western Mediterranean endemic dragonfly Orthetrum nitidinerve (Selys, 1841): insights from Italy

Aquatic macroinvertebrates are a primary component of freshwater ecosystems and one of the most threatened by anthropogenic pressures. Among them, dragonflies are a charismatic group of growing scientific and social interest. However, little is known about the natural history of several species. One paradigmatic example is the declining Orthetrum nitidinerve, a Western Mediterranean endemic anisopteran….

Out of Australia: the Argiolestidae reveal the Melanesian Arc System and East Papua Composite Terrane as possible ancient dispersal routes to the Indo-Australian Archipelago (Odonata: Argiolestidae)

Information on the origin of distribution patterns shown by freshwater invertebrates in the Indo-Australian Archipelago is poor. Here we present a molecular based hypothesis of the phylogenetic relationships of Argiolestidae, a family of damselflies found throughout the tropical parts of the Eastern Hemisphere. We use this to address the following questions: (1) did Argiolestidae colonize…

The complete mitochondrial genome of the broad-winged damselfly Mnais costalis Selys (Odonata: Calopterygidae) obtained by next-generation sequencing

We used next-generation sequencing to characterise the complete mitochondrial genome of the damselfly Mnais costalis (Odonata, Calopterygidae). Illumina paired end reads were mapped against COI and 16S sequences from M. costalis and then extended using an iterative de novo map procedure. The final assembly was a contiguous sequence of 15,487 bp, which contained all standard mitochondrial…

New EPIC nuclear DNA sequence markers to improve the resolution of phylogeographic studies of coenagrionids and other odonates

While phylogeographic data provide valuable information to inform conservation plans, there are comparatively few Odonata phylogeographic studies. This lack of research is partially due to a lack of independent DNA markers with appropriate levels of polymorphism that PCR-amplify in a range of species. We followed an exon-primed, intron-crossing (EPIC) PCR strategy to develop five new,…

Pyrrhosoma and its relatives: a phylogenetic study (Odonata: Zygoptera)

The placement and relationships of the red-and-black zygopteran Pyrrhosoma, currently considered to be part of the Teinobasinae, has long been uncertain. DNA fragments (COI and ITS) reveal that Pyrrhosoma s.s. is restricted to the West Palaearctic, with two morphologically distinct name-bearing clades (nymphula, elisabethae), and with a morphologically indistinct third clade in the Middle Atlas,…