New EPIC nuclear DNA sequence markers to improve the resolution of phylogeographic studies of coenagrionids and other odonates

While phylogeographic data provide valuable information to inform conservation plans, there are comparatively few Odonata phylogeographic studies. This lack of research is partially due to a lack of independent DNA markers with appropriate levels of polymorphism that PCR-amplify in a range of species. We followed an exon-primed, intron-crossing (EPIC) PCR strategy to develop five new,…

Distribution, autecology, genetic characterization, and conservation of the Western Mediterranean endemic dragonfly Orthetrum nitidinerve (Selys, 1841): insights from Italy

Aquatic macroinvertebrates are a primary component of freshwater ecosystems and one of the most threatened by anthropogenic pressures. Among them, dragonflies are a charismatic group of growing scientific and social interest. However, little is known about the natural history of several species. One paradigmatic example is the declining Orthetrum nitidinerve, a Western Mediterranean endemic anisopteran….

Microneura is a junior synonym of Protoneura (Zygoptera, Coenagrionidae)

Microneura caligata (Hagen in Selys, 1886) is an endangered damselfly presently known from five localities in the central mountains of Cuba. The precise systematic position of this species within the former Neotropical Protoneuridae has been the subject of debate, with previous results from a phylogenetic analysis based on morphology suggesting that the genus Microneura should…

Morphometric and molecular studies on the populations of the damselflies Chalcolestes viridis and C. parvidens (Odonata, Lestidae)

Morphometric and genetic differences were analysed for two closely related damselflies, Chalcolestes viridis and C. parvidens. A total of 305 male individuals were collected from six European countries (Austria, Croatia, Germany, Greece, Hungary and Portugal). Measurements from a total of 28 populations of C. viridis and C. parvidens and several intermediate forms were collected to…

Dragons fly, biologists classify: an overview of molecular odonate studies, and our evolutionary understanding of dragonfly and damselfly (Insecta: Odonata) behavior

Among insects, perhaps the most appreciated are those that are esthetically pleasing: few capture the interest of the public as much as vibrantly colored dragonflies and damselflies (Insecta: Odonata). These remarkable insects are also extensively studied. Here, we review the history of odonate systematics, with an emphasis on discrepancies among studies. Over the past century,…