The Bekko Tombo, Libellula angelina Selys, 1883 (Odonata: Libellulidae), is listed as an endangered species in South Korea, and is classified as a critically endangered species by the International Union for Conservation of Nature (IUCN). An assessment of the genetic diversity and population relationships of the species by molecular markers can provide the information necessary…
We isolated and characterized a total of 13 microsatellite loci from Cordulegaster sarracenia (Odonata: Cordulegastridae). Loci were screened in 24 individuals from Louisiana and Texas. Within C. sarracenia, the number of alleles per locus ranged from 0 to 5, and observed and expected heterozygosities ranged from 0.000 to 0.556 and 0.000 to 0.613, respectively. Overall
Pantala flavescens (Fabricius, 1798) is one of the most common species of dragonflies and has been found throughout from tropic to temperate zones worldwide. In this study, RNA-seq of P. flavescens was carried out through Illumina high-throughput sequencing technology. Approximately 37,868 unigenes and 47,188 transcripts were obtained. The average length of the assembled unigenes was…
While phylogeographic data provide valuable information to inform conservation plans, there are comparatively few Odonata phylogeographic studies. This lack of research is partially due to a lack of independent DNA markers with appropriate levels of polymorphism that PCR-amplify in a range of species. We followed an exon-primed, intron-crossing (EPIC) PCR strategy to develop five new,…
Microsatellite loci have become the genetic markers of choice for population-level molecular ecological studies. However, microsatellite loci had been isolated for comparatively few species of odonate until the past five years. This review summarises the main characteristics – expected heterozygosity and microsatellite length – of 116 microsatellite loci that have been isolated from the genomes…
The taxonomic status of the Central Asian damselfly Calopteryx samarcandica was determined on the basis of molecular and phenotypic data from the Kugitang Mountains, SE Uzbekistan.
Within Europe, the damselfly Coenagrion hylas has a very limited distribution and is regarded as a vulnerable species. For studying migration and population connectivity in the Central European populations, 10 microsatellite markers were developed for this species.
Microsatellites have been infrequently used for genetic analysis of odonate species. Here, we report four microsatellite loci that are polymorphic in Coenagrion mercuriale. Furthermore, we examine the success of cross-species amplification of a panel of 19 microsatellite loci that were developed from C. mercuriale in seven other European odonate species. PCR with microsatellite primers is…