Development and validation of microsatellite markers for an endangered dragonfly, Libellula angelina (Odonata: Libellulidae), with notes on population structures and genetic diversity

The Bekko Tombo, Libellula angelina Selys, 1883 (Odonata: Libellulidae), is listed as an endangered species in South Korea, and is classified as a critically endangered species by the International Union for Conservation of Nature (IUCN). An assessment of the genetic diversity and population relationships of the species by molecular markers can provide the information necessary…

Development of SSR markers based on the head transcriptome of Pantala flavescens (Fabricius, 1798) (Odonata: Libellulidae)

Pantala flavescens (Fabricius, 1798) is one of the most common species of dragonflies and has been found throughout from tropic to temperate zones worldwide. In this study, RNA-seq of P. flavescens was carried out through Illumina high-throughput sequencing technology. Approximately 37,868 unigenes and 47,188 transcripts were obtained. The average length of the assembled unigenes was…

Development of polymorphic microsatellite markers for a rare dragonfly, Cordulegaster sarracenia (Odonata: Cordulegastridae), with notes on population structure and genetic diversity

We isolated and characterized a total of 13 microsatellite loci from Cordulegaster sarracenia (Odonata: Cordulegastridae). Loci were screened in 24 individuals from Louisiana and Texas. Within C. sarracenia, the number of alleles per locus ranged from 0 to 5, and observed and expected heterozygosities ranged from 0.000 to 0.556 and 0.000 to 0.613, respectively. Overall

The complete mitochondrial genome of the broad-winged damselfly Mnais costalis Selys (Odonata: Calopterygidae) obtained by next-generation sequencing

We used next-generation sequencing to characterise the complete mitochondrial genome of the damselfly Mnais costalis (Odonata, Calopterygidae). Illumina paired end reads were mapped against COI and 16S sequences from M. costalis and then extended using an iterative de novo map procedure. The final assembly was a contiguous sequence of 15,487 bp, which contained all standard mitochondrial…

Redescription of the holotype of Mecistogaster pronoti Sjöstedt, 1918 (Zygoptera: Pseudostigmatidae)

Mecistogaster pronoti Sjöstedt, 1918 was described based on a female holotype deposited in the Naturhistoriska Riksmuseet, Stockholm, collected in the state of Espírito Santo, Brazil. The original description has no illustrations, which makes its identification very difficult. Herewith we redescribe and illustrate this holotype. The species is red listed and considerations regarding its conservation are…

Territorial behaviour associated with feeding in both sexes of the tropical zygopteran, Libellago hyalina (Odonata: Chlorocyphidae)

Territorial behaviour associated with adult odonate feeding in a Bornean rain forest under-storey is described and its underlying causes are analysed. Immature males and females of all ages of Libellago hyalina defended perches along a narrow trail in kerangas forest, concentrating especially around patches of sunlight. Throughout the day there was displacement of individuals as…