New EPIC nuclear DNA sequence markers to improve the resolution of phylogeographic studies of coenagrionids and other odonates

While phylogeographic data provide valuable information to inform conservation plans, there are comparatively few Odonata phylogeographic studies. This lack of research is partially due to a lack of independent DNA markers with appropriate levels of polymorphism that PCR-amplify in a range of species. We followed an exon-primed, intron-crossing (EPIC) PCR strategy to develop five new,…

Development of SSR markers based on the head transcriptome of Pantala flavescens (Fabricius, 1798) (Odonata: Libellulidae)

Pantala flavescens (Fabricius, 1798) is one of the most common species of dragonflies and has been found throughout from tropic to temperate zones worldwide. In this study, RNA-seq of P. flavescens was carried out through Illumina high-throughput sequencing technology. Approximately 37,868 unigenes and 47,188 transcripts were obtained. The average length of the assembled unigenes was…

Microneura is a junior synonym of Protoneura (Zygoptera, Coenagrionidae)

Microneura caligata (Hagen in Selys, 1886) is an endangered damselfly presently known from five localities in the central mountains of Cuba. The precise systematic position of this species within the former Neotropical Protoneuridae has been the subject of debate, with previous results from a phylogenetic analysis based on morphology suggesting that the genus Microneura should…